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Ethylestrenol is a mild AAS, having very little anabolic or androgenic effect at therapeutic dosesand little or no ability to induce estrogenic-like responses in normal animals. In animal studies, the major metabolite of E1 is D2. However, in some cases, the metabolism of estrogens into estrogenic metabolites (e.g. EE2) does not occur. It has been shown that anabolic steroids can be metabolized into the non-steroidal androgen dihydrotestosterone by 3-hydroxyisovaleric acid dehydrogenase, and by esterase enzymes (1), which results in a more pronounced androgen effect.
This metabolite, EE2, may induce more potent androgenic effects than E1. Estrogen-related effects in animals with low levels of DHT and/or E2 or in animals which lack a functional estrogen receptor have been reported, although there is some controversy as to how these effects could be mediated (2,3). However, a study of the effect of DHT or E2 on the sexual response and Sertoli Cells in rats has been questioned because of the high concentrations of DHT which could have an adverse effect on reproductive systems (4).
This study was designed to investigate the effects of E1 and EE2 on the sexual arousal and Sertoli Cells in rats. The study was conducted in accordance with Good Laboratory Practice guidelines. The primary outcome measures were the degree of sexual arousal and the number and extent of Sertoli Cells. Secondary measures included the number of estrogens present in the plasma and on the Sertoli Cells; number of androgen receptors present on the Sertoli Cells; number of androgen receptors present on the cells; as well as the effects of DHT and/or E2 on these parameters. Additionally, we attempted to determine the effects of different pharmacological agents on the sexual performance and Sertoli cells.
METHODS
Study subjects
Nine male Sprague–Dawley rats of each sex were obtained from Harlan Laboratories (Bristol , England). Each animal was housed in a pair and used under the same conditions as used in the study mentioned above. These rats had a body weight of 60–65 g. Rats were housed in individual cages and fed Standard chow for 10 weeks before the experiment. These rats had been previously used in studies of sex steroid binding (5–7).
Instruments
Male rats were anesthetized with isoflurane (2.5%/vol) in 0.1 L
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